Mathematical Modeling: Bridging the Gap between Concept and Realization in Synthetic Biology
Figure 8
The six possible binding states in the repressor-activator model in Guido et al. [8]. The rectangular boxes represent the three available operator sites of engineered promoter. Unbound sites are depicted with unfilled (white) boxes; filled (pink) boxes depict sites bound by the appropriate protein (lacI to , cI to 1 and 2). Of the eight () possible states, two are not feasible. This is because cI binds sequentially to the 1 and 2 sites so that 2 cannot bind unless 1 is also bound. The model parameters through are the rate constants for the transitions between each possible binding state (adapted from Guido et al. [8]).