Research Article

The Adenovirus Type 3 Dodecahedron's RGD Loop Comprises an HSPG Binding Site That Influences Integrin Binding

Figure 4

FACS analysis of the dp8 effect on Bs-Dd binding to HSPG deficient CHO-2241 cells in presence or absence of calcium. Bs-Dd was incubated at two different concentrations with CHO-2241 cells at 4 C in PBS supplemented or not with 1 mM CaC l 2 (upper and lower panels, respectively). Bound Bs-Dd alone (green curves), or preincubated with dp8 oligosaccharide (purple curves), was detected by an antibody serum directed against the HAd3 penton base.