Increasing a Robust Antigen-Specific Cytotoxic T Lymphocyte Response by FMDV DNA Vaccination with IL-9 Expressing Construct
Expression of apoptotic or proapoptotic genes in CD8+ T cells by RT-PCR. Total RNA was isolated from the splenic CD8+ T cells of immunized on day 7 after final boost. (a) The expression levels of apoptotic or proapoptotic genes were semiquantitatively measured by RT-PCR through the normalization of the tested cDNA concentration to the amount of -actin. (b), (c), (d), (e) the density of each band as determined using Alpha image software and expressed as a relative intensity against the -actin band. From left to right, 1, naïve; 2, pcD/proV; 3, proV-IL9/pcD; 4, pcD-VP1/proV; 5, pcD-VP1/proV-IL9.
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