Two-Dimensional Liquid Chromatography Technique Coupled with Mass Spectrometry Analysis to Compare the Proteomic Response to Cadmium Stress in Plants
Figure 3
A “ProteoVue” 2D map of a leaf protein extract from Populus nigra clone “Poli”, grown for 3 weeks in nutrient solution supplemented with 50 M CdSO4. The -axis is in isoelectric point (pI) units from 4.0 to 8.0. The -axis displays increasing hydrophobicity. The color scale of the bands represents the relative intensity of each band by UV detection at 214 nm. The proteins detected by MALDI/TOF analysis are numbered and highlighted with yellow arrows.
