Organogenic Nodule Formation in Hop: A Tool to Study Morphogenesis in Plants with Biotechnological and Medicinal Applications
Figure 2
(a) Detection of MBP Kinase Activity in hop extracts at different time points (T0, T1, T3, T5, T10, T20, T30, and T60 min) immediately after wounding. In in-gel kinase assays MAPK phosphorylates MBP. A band of approximately 50 kDa was induced upon wounding of hop internodes. (b) MAPK 6 activity during hop organogenesis (T0, T2h, T12h, T24h, T48h, T4d, T7d, T15d, T28d, and T45d). Phosphorylation of MBP was analyzed by autoradiography following immunoprecipitation with MAPK6 antibody. In hop extracts and Arabidopsis (lanes Arab) subjected to in-gel kinase assay, a band of approximately 46-kDa was detected.
