Intranasal Immunization with Chitosan/pCAGGS-flaA Nanoparticles Inhibits Campylobacter jejuni in a White Leghorn Model

Figure 2

Protection of DNA from DNAse I digestion by chitosan. Naked DNA and chitosan-DNA complex containing 1 𝜇g DNA were both incubated with DNAse I for 15 min at 37^∘C. Then the reaction was stopped by adding with 0.5 M EDTA. chitosan-DNA was then subjected to chitosanase and lysozyme digestion. Finally, all samples were run on 0.8% (w/v) agarose gel and stained with ethidium bromide. Lane 1: chitosan-DNA; 2: chitosan-DNA + DNAse I (28 U/mL); 3: chitosan-DNA + chitosanase (0.15 U/mL) + lysozyme (15 U/mL); 4: 3 + DNAse I (28 U/mL); 5: DNA+ DNAse I (28 U/mL); 6: DNA (pCAGGS-flaA, 4.1 kb).