Mitoxantrone Loaded Superparamagnetic Nanoparticles for Drug Targeting: A Versatile and Sensitive Method for Quantification of Drug Enrichment in Rabbit Tissues Using HPLC-UV

Figure 2

Extraction efficiency: extraction with: 500 𝜇L water, 200 𝜇L MeOH, 50 𝜇L ascorbic acid (20%) in citrate buffer (pH 3.0), 200 𝜇L formic acid, 100 𝜇L 20% trichloroacetic acid, and 400 𝜇L chloroform in an ultrasonic bath. Recovery rate in dependence to multiple extractions. 4 fold extraction is nearly quantitative. Each reading point was measured in triplicate.