BioMed Research International

Methodology Report

Mitoxantrone Loaded Superparamagnetic Nanoparticles for Drug Targeting: A Versatile and Sensitive Method for Quantification of Drug Enrichment in Rabbit Tissues Using HPLC-UV

Table 1

Extraction of porcine muscle and liver tissues containing pure mitoxantrone (MTO) or MTO bound to ferrofluid (FF). The Table shows the high recovery rate of pure MTO and a lower recovery rate of FF-bound MTO in different tissues.
SampleExtraction modality (1)Recovery [%] (2)
2 g liver/100  𝜇 g MTO 2 × MeOH: 1 N HCl = 50 : 50 9 6 ± 1 0
1 g muscle tissue/100  𝜇 g MTO 2 × 0 , 5 HCl 7 4 ( 3 )
1 g muscle tissue/100  𝜇 g MTO 2 × MeCN: 0,5 N HCl 10 : 90 8 5 ± 9
1 g muscle tissue/100  𝜇 g MTO 2 × MeOH: 1 N HCl = 50 : 50 8 7 ± 9
2 g muscle tissue/100  𝜇 g MTO + 500  𝜇 L FF 2 × MeOH: 1 N HCl = 50 : 50 6 4 ± 7
1 g muscle tissue/100  𝜇 g MTO + 500  𝜇 L FF 2 × MeCN: 0,5 N HCl 10 : 90 7 9 ± 8
2.5 g liver/100  𝜇 g MTO + 1 mL FF 2 × MeOH: 1 N HCl = 50 : 50 5 6 ± 2 7
2.5 g liver/100  𝜇 g MTO + 1 mL FF 2 × MeCN: 1 N HCl = 10 : 90 5 8 ± 1 5
(1)Typical extraction procedure: double incubation in ultrasonic bath, each for 10 minutes. Collecting the supernatant after centrifugation and HPLC-measurement afterwards.
(2)number of independent experiments 𝑛 = 3
(3)single experiment.