Mitoxantrone Loaded Superparamagnetic Nanoparticles for Drug Targeting: A Versatile and Sensitive Method for Quantification of Drug Enrichment in Rabbit Tissues Using HPLC-UV

Table 2

Comparison of different extraction techniques.

Matrix^(1)	Extraction mode^(2)	Recovery [%]^(3)
MTO + 0.5 g porcine liver	1 fold vortexing 3 minutes	22±4
FF-MTO + 0.5 g porcine liver	1 fold vortexing 3 minutes	26±4
FF-MTO + 0.5 g porcine liver	1 fold vortexing 3 minutes + 5 minutes Ultrasound	34±3
FF-MTO + 0.5 g porcine liver	4 hours shaking (Thermomixer Eppendorf Comfort 600 rpm)	27±2
FF-MTO + 0.5 g porcine liver	24 hours shaking (Thermomixer Eppendorf Comfort 600 rpm)	18^(4)
FF-MTO + 0.5 g porcine liver	1×1 hour Ultrasound	38±3
FF-MTO + 0.5 g porcine liver	1×4 hours Ultrasound	31±5
FF-MTO + 0.5 g porcine liver	4×1 hour Ultrasound	76±6
FF-MTO + 5 g porcine liver	ASE^(5) 40°C, 1 cycle	36±6
FF-MTO + 5 g porcine liver	ASE^(5) 80°C, 1 cycle	81±6

⁽¹⁾MTO-amount added to the tissue homogenate samples: 25 𝜇g MTO, ferrofluid amount (FF): 1 mL
⁽²⁾Extraction solution: 500 𝜇L water, 50 𝜇L ascorbic acid (20%) in citrate buffer (pH 3.0), 200 𝜇L MeOH, 200 𝜇L formic acid, 100 𝜇L 20% trichloroacetic acid, 400 𝜇L chloroform.
⁽³⁾number of independent experiments 𝑛=3 performed on 3 different days
⁽⁴⁾single experiment
⁽⁵⁾ASE= Accelerated solvent extraction: solvent composition: 20% (v/v) MeOH, 20% (v/v) formic acid, 10% (v/v) trichloroacetic acid (20%), 50% (v/v) chloroform.