BioMed Research International

Methodology Report

Mitoxantrone Loaded Superparamagnetic Nanoparticles for Drug Targeting: A Versatile and Sensitive Method for Quantification of Drug Enrichment in Rabbit Tissues Using HPLC-UV

Table 3

Mitoxantrone (MTO) recovery of pure MTO or MTO bound to ferrofluid (FF) in 2.5 g tissue using a RP-18-Cartridge. 1st section shows extractants based on hydrochloric acid, 2nd section shows phosphoric acid-based extractants, and 3rd section shows phosphate-buffer extractants. In general: FF-bound MTO in liver tissue exhibited insufficient recovery rates.
CartridgeMatrixExtraction mode (3)Condition/Elution-modeRecovery [%](1)
No cartridgeFF-MTO1 N HClNo cartridge93(2)
Sep-Pak-Plus C18MTO1 N HCl1 N HCl/MeOH 7 7 ± 6
1stSep-Pak-Plus C18MTO + porcine muscle tissue1 N HClH2O/MeOH 6 7 ± 2
Sep-Pak-Plus C18MTO + porcine liver1 N HCl1 N HCl/MeOH 4 1 ± 1 1
Sep-Pak-Plus C18FF-MTO1 N HCl1 N HCl/MeOH 4 ± 1
Sep-Pak-Plus C18FF-MTO + porcine liver1 N HCl1 N HCl/MeOH 9 ± 8
No CartridgeFF-MTO0.1 M H3PO4No Cartridge94(2)
2ndSep-Pak-Plus C18FF-MTO0.1 M H3PO41 N HCl/MeOH 7 4 ± 1 0
Sep-Pak-Plus C18MTO + porcine liver0.1 M H3PO40,1 M H3PO4/MeOH 7 6 ± 1 0
Sep-Pak-Plus C18FF-MTO + porcine liver0.1 M H3PO4MeOH/0, 1 M H3PO4 1 2 ± 8
3rdSep-Pak-Plus C18FF-MTO + porcine liver0.1 M KH2PO4/H3PO4 (pH = 6.0)1 N HCl/MeOHn.d.(4)
Sep-Pak-Plus C18FF-MTO + porcine liver0.1 M KH2PO4/HCl1 N HCl/MeOH 1 7 ± 1 6
(1)number of independent experiments = 3
(2)single experiment
(3)Typical extraction procedure: double incubation in ultrasonic bath each for 10 minutes. Collecting the supernatant after centrifugation and HPLC-measurement afterwards
(4)Not detectable.