Dynamic Metabolic Flux Analysis Demonstrated on Cultures Where the Limiting Substrate Is Changed from Carbon to Nitrogen and Vice Versa

Figure 2

Two ways of estimating the derivative in point 𝑎: 𝐷₁ is calculated by symbolically deriving the function in point 𝑎; 𝐷₂ is the slope between point 𝑎 and point 𝑏 (where 𝑎 and 𝑏 are calculated from the curve). Dots represent sample points through which a curve is fitted.