Ordered Assembly of the Adhesive and Electrochemical Connections within Newly Formed Intercalated Disks in Primary Cultures of Adult Rat Cardiomyocytes
Figure 5
Localization of plakoglobin and plakophilin-2 in remodeling adult rat cardiac myocytes. ARCs were immunolabeled with antibodies to plakoglobin (PG) (red: a, b, d, e) and PKP2 (green: a, c, d, f) on day 5 post plating. Note the extensive co-localization of the two armadillo family proteins to new cell adhesion domains between contacting cells (a)–(c). There was some regional variation in the relative abundance of the two such that some newly-formed contacts contained predominately plakoglobin ((e): arrow; (e)-inset: asterisks) or PKP2 ((f) and (f)-inset: arrowheads). Scale bars are 20 m and the insets in (e) and (f) are a 2 magnification of a region of cell-cell contact.
