Ordered Assembly of the Adhesive and Electrochemical Connections within Newly Formed Intercalated Disks in Primary Cultures of Adult Rat Cardiomyocytes
Figure 6
Localization of DP to a subset of adhesive sites. ARCs were immunolabeled with antibodies to desmoplakin (red: a, b) and PKP-2 (green: a, c) on days 5 (a)–(c) and 7 (d)–(f) post plating. The absence of DP from some of the adhesive domains ((a)–(c): arrowheads) suggests that its localization to the newly forming structures occurs after PKP2. DP and PKP2 do fully co-localize at more mature adhesive contacts ((d)–(f): arrowheads). Scale bars are 10 m (a)–(c) and 20 m (e, f).
