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Journal of Biomedicine and Biotechnology
Volume 2010, Article ID 630267, 8 pages
Research Article

Short-Term Evaluation in Growing Rats of Diet Containing Bacillus thuringiensis Cry1Ia12 Entomotoxin: Nutritional Responses and Some Safety Aspects

1Departamento de Biotecnologia, Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF 70.770-900, Brazil
2Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará 60.451-970, Brazil
3Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, Ceará 60.740-000, Brazil
4Departamento de Biologia, Universidade Federal do Ceará, Fortaleza, Ceará 60.455-970, Brazil
5Departamento de Medicina Veterinária, União Pioneira de Integração Social, Planaltina, DF 70.390-125, Brazil
6Departamento de Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, DF 70.790-160, Brazil

Received 4 April 2010; Revised 3 August 2010; Accepted 11 August 2010

Academic Editor: Roy Gross

Copyright © 2010 Luciane Mourão Guimarães et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The Cry1Ia12 entomotoxin from a Brazilian Bacillus thuringiensis strain is currently being expressed in cotton cultivars to confer resistance to insect-pests. The present study aimed to assess the effects of a diet containing Cry1Ia12 protein on growing rats. A test diet containing egg white and Cry1Ia12 (0.1% of total protein) as a protein source was offered to rats for ten days. In addition, an acute toxicity bioassay was performed in rats with a single oral dose of the entomotoxin (12 mg/animal). No adverse effects were observed in the animals receiving the test diet when compared to those receiving a control diet (egg white). The analysed parameters included relative dry weight of internal organs, duodenum histology, blood biochemistry, and nutritional parameters. The results of the acute toxicity test showed no mortality or behaviour alteration. Thus, Cry1Ia12 toxin at the tested concentration does not cause deleterious effects on growing rats when incorporated in the diet for 10 days.