Proteomic Profiling of the Dystrophin-Deficient MDX Heart Reveals Drastically Altered Levels of Key Metabolic and Contractile Proteins

Table 1

List of DIGE-identified proteins with a changed abundance in 9-month old dystrophic MDX heart muscle.

Spot No.	Protein name	Protein ID	Peptides matched	Coverage (%)	Molecular mass (kDa)	Isoelectric point (pI)	Mascot score	Fold change
1	Nucleoside diphosphate kinase B	NDKB_MOUSE	4	34	17.5	6.9	69	4.4
2	Lamin-A/C	LMNA_MOUSE	4	6	74.5	6.5	111	1.9
3	Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial	ETFD_MOUSE	8	15	68.9	7.3	45	1.8
4	Myozenin-2	MYOZ2_MOUSE	4	20	29.8	8.5	145	0.67
5	Electron transfer flavoprotein subunit alpha, mitochondrial	ETFA_MOUSE	2	12	35.3	8.6	89	0.67
6	Prohibitin	PHB_MOUSE	8	41	29.9	5.6	290	0.67
7	Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial	ODO2_MOUSE	3	6	49.3	9.1	86	0.63
8	Isovaleryl-CoA dehydrogenase, mitochondrial	IVD_MOUSE	7	23	46.7	8.5	89	0.59
9	Serum albumin	ALBU_MOUSE	3	5	70.7	5.8	82	0.59
10	Cytochrome b-c1 complex subunit Rieske, mitochondrial	UCRI_MOUSE	4	13	29.6	8.9	84	0.56
11	Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial	ODP2_MOUSE	10	20	68.5	8.8	117	0.53
12	Vimentin	VIME_MOUSE	11	20	53.7	5.1	118	0.53
13	Beta-enolase	ENOB_MOUSE	8	19	47.3	6.7	111	0.50
14	Pyruvate dehydrogenase E1 component subunit beta, mitochondrial	ODPB_MOUSE	8	31	39.3	6.4	279	0.50
15	Pyruvate dehydrogenase E1 component subunit beta, mitochondrial	ODPB_MOUSE	3	19	39.3	6.4	134	0.50
16	Creatine kinase M-type	KCRM_MOUSE	2	4	43.3	6.6	47	0.50
17	Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial	ECH1_MOUSE	4	24	36.4	7.6	201	0.50
18	Heat shock protein beta-1 Hsp27	HSPB1_MOUSE	2	8	23.1	6.1	50	0.50
19	Isovaleryl-CoA dehydrogenase, mitochondrial	IVD_MOUSE	7	17	46.7	8.5	98	0.50
20	Pyruvate dehydrogenase protein X component, mitochondrial	ODPX_MOUSE	5	11	54.3	7.6	63	0.48
21	Actin, alpha cardiac muscle 1	ACTC_MOUSE	6	19	42.3	5.2	104	0.48
22	Cytochrome c oxidase subunit 5B, mitochondrial	COX5A_MOUSE	2	14	14.1	8.7	52	0.48
23	Vimentin	VIME_MOUSE	13	27	53.7	5.1	122	0.48
24	Transitional endoplasmic reticulum ATPase (valosin-containing protein VCP)	TERA_MOUSE	5	10	89.9	5.1	171	0.46
25	Pyruvate dehydrogenase E1 component subunit beta, mitochondrial	ODPB_MOUSE	4	14	39.3	6.4	100	0.44
26	Myosin regulatory light chain 2, ventricular/cardiac muscle isoform	MLRV_MOUSE	9	52	18.9	4.9	209	0.44
27	Fatty acid-binding protein FABP-3, heart	FABPH_MOUSE	5	37	14.8	6.1	149	0.44
28	Enolase 1, alpha	ENOA_MOUSE	2	3	47.5	6.4	43	0.44
29	DJ-1 protein	PARK7_MOUSE	4	26	20.2	6.3	90	0.44
30	Cytochrome b-c1 complex	QCR1_MOUSE	6	17	53.4	5.8	83	0.44
31	60 kDa heat shock protein, Hsp60, mitochondrial	CH60_MOUSE	2	6	61.1	5.9	63	0.42
32	Beta-enolase	ENOB_MOUSE	3	7	47.3	6.7	92	0.42
33	Fatty acid binding protein, FABP-3, heart	FABPH_MOUSE	7	52	14.8	6.1	162	0.42
34	Pyruvate dehydrogenase protein X component, mitochondrial	ODPX_MOUSE	2	4	54.3	7.6	66	0.42
35	Tropomyosin alpha-1 chain	TPM1_MOUSE	15	43	32.7	4.7	302	0.40
36	Electron transfer flavoprotein subunit alpha, mitochondrial	ETFA_MOUSE	8	33	35.3	8.6	235	0.40
37	60 kDa heat shock protein, Hsp60, mitochondrial	CH60_MOUSE	12	32	61.1	5.9	346	0.40
38	Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial	IDH3A_MOUSE	9	29	40.1	6.3	164	0.40
39	Actin, alpha cardiac muscle 1	ACTC_MOUSE	12	31	42.3	5.2	317	0.39
40	NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial	NDUV2_MOUSE	6	29	27.6	7.0	370	0.39
41	Voltage-dependent anion-selective channel protein VDAC1	VDAC1_MOUSE	10	29	32.5	8.6	292	0.39
42	Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial	IDH3A_MOUSE	13	39	40.1	6.3	376	0.39
43	Desmin	DESM_MOUSE	30	50	53.5	5.2	1184	0.39
44	60 kDa heat shock protein, Hsp60, mitochondrial	CH60_MOUSE	25	38	61.1	5.9	842	0.37
45	Actin, alpha cardiac muscle 1	ACTC_MOUSE	10	26	42.3	5.2	276	0.36
46	Actin, alpha cardiac muscle 1	ACTC_MOUSE	4	14	42.3	5.2	88	0.36
47	Desmin	DESM_MOUSE	15	37	53.5	5.2	349	0.36
48	Myosin regulatory light chain 2, atrial isoform	MLRA_MOUSE	9	57	19.6	4.8	299	0.36
49	Fatty acid-binding protein, FABP-3, heart	FABPH_MOUSE	12	61	14.8	6.1	425	0.35
50	ATP synthase subunit beta, mitochondrial	ATPB_MOUSE	18	40	56.3	5.2	1093	0.35
51	Cytochrome c oxidase subunit 5A	COX5A_MOUSE	7	28	16.3	6.1	286	0.35
52	Desmin	DESM_MOUSE	26	62	53.5	5.2	1657	0.35
53	Myosin light chain 3	MYL3_MOUSE	6	41	22.5	5.0	90	0.35
54	Desmin	DESM_MOUSE	12	30	53.5	5.2	431	0.33
55	ATP synthase subunit d, mitochondrial	ATP5H_MOUSE	3	21	18.8	5.5	48	0.33
56	Actin, alpha cardiac muscle 1	ACTC_MOUSE	16	40	42.3	5.2	598	0.32
57	Cytochrome b-c1 complex	QCR1_MOUSE	12	27	53.4	5.8	330	0.32
58	Desmin	DESM_MOUSE	36	68	53.5	5.2	1289	0.32
59	Tropomyosin alpha-1 chain	TPM1_MOUSE	21	46	32.7	4.7	605	0.32
60	Actin, alpha cardiac muscle 1	ACTC_MOUSE	18	59	42.3	5.2	450	0.31
61	Myosin light chain 3	MYL3_MOUSE	11	63	22.5	5.0	231	0.30
62	Myosin regulatory light chain 2, ventricular/cardiac muscle isoform	MLRV_MOUSE	13	73	18.9	4.9	118	0.29
63	Cytochrome b-c1 complex	QCR1_MOUSE	12	31	53.4	5.8	342	0.29
64	Actin, alpha cardiac muscle 1	ACTC_MOUSE	7	36	42.3	5.2	160	0.29
65	Myosin regulatory light chain 2, ventricular/cardiac muscle isoform	MLRV_MOUSE	7	46	18.9	4.9	354	0.29
66	Actin, alpha cardiac muscle 1	ACTC_MOUSE	4	24	42.3	5.2	91	0.28
67	Actin, alpha cardiac muscle 1	ACTC_MOUSE	17	62	42.3	5.2	294	0.27
68	Adenylate kinase isoenzyme 1	KAD1_MOUSE	5	31	21.6	5.7	174	0.26
69	ATP synthase subunit beta, mitochondrial	ATPB_MOUSE	4	21	56.3	5.2	92	0.26
70	Myosin regulatory light chain 2	MLRA_MOUSE	6	47	19.6	4.8	168	0.25
71	Actin, alpha cardiac muscle 1	ACTC_MOUSE	18	61	42.3	5.2	302	0.25
72	ATP synthase subunit d, mitochondrial	ATP5H_MOUSE	5	36	18.8	5.5	69	0.25
73	ATP synthase subunit beta, mitochondrial	ATPB_MOUSE	18	51	56.3	5.2	640	0.25
74	ATP synthase subunit beta, mitochondrial	ATPB_MOUSE	25	71	56.3	5.2	1102	0.25
75	Actin, alpha cardiac muscle 1	ACTC_MOUSE	6	16	42.3	5.2	119	0.25
76	Myosin light chain 3	MYL3_MOUSE	6	30	22.5	5.0	99	0.25
77	ATP synthase subunit beta, mitochondrial	ATPB_MOUSE	24	70	56.3	5.2	871	0.24
78	Myosin light chain 2, ventricular/cardiac	MLRV_MOUSE	11	77	18.9	4.9	206	0.24
79	Peroxiredoxin-6	PRDX6_MOUSE	3	19	24.9	5.7	102	0.19