Cloning, Purification, and Partial Characterization of Bacillus subtilis Urate Oxidase Expressed in Escherichia coli
Figure 1
Small-scale expression and time course study of urate oxidase expression in E. coli. Comassie Blue stained 12% SDS-PAGE (a) and respective Western blotting analysis (b) of urate oxidase induction in E. coli. Lane M, protein BenchMarker (Invitrogen); Lane 1, supernatant of uninduced cells; Lanes 2–6, supernatant of induced cells after 15, 30, 60, 90, and 120 minutes, respectively. The arrow indicates the position of the band corresponding to recombinant urate oxidase (rUox).
