Cloning, Purification, and Partial Characterization of <i>Bacillus subtilis</i> Urate Oxidase Expressed in <i>Escherichia coli</i>

<table>Recombinant enzyme purification. Fractions collected before and after Ni-NTA chromatography were visualized on 12% SDS-PAGE. Lane M, protein BenchMarker (Invitrogen); Lane 1, crude extract of uninduced <i>E. coli</i> cells; Lane 2, crude cell extract after induction for 3 hours; Lane 3, cellular extract (input); Lane 4, flow-through; Lane 5, washing step (20 mM imidazole); Lane 6, purified urate oxidase (<svg height="5.7874999" id="M35" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 10.825 5.7874999" width="10.825" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,5.7875)">
<g transform="translate(72,-67.37)">
<text transform="matrix(1,0,0,-1,-71.95,67.42)">
<tspan style="font-size: 12.50px; " x="0" y="0">∼</tspan>
</text>
</g>
</g>
</svg>30.5 <svg height="9.6750002" id="M36" style="vertical-align:-2.29482pt" version="1.1" viewbox="0 0 9.6374998 9.6750002" width="9.6374998" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,9.675)">
<g transform="translate(72,-64.26)">
<text transform="matrix(1,0,0,-1,-71.95,66.6)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝜇</tspan>
</text>
</g>
</g>
</svg>g) after elution with 200 mM imidazole.</table>

BioMed Research International

fig2

Figure 2

Figure 2: Cloning, Purification, and Partial Characterization of <i>Bacillus subtilis</i> Urate Oxidase Expressed in <i>Escherichia coli</i> 

BioMed Research International

Cloning, Purification, and Partial Characterization of Bacillus subtilis Urate Oxidase Expressed in Escherichia coli

Figure 2