Cloning, Purification, and Partial Characterization of Bacillus subtilis Urate Oxidase Expressed in Escherichia coli

Figure 3

Mass spectometry. MALDI-TOF/MS spectrum of recombinant urate oxidase using sinapinic acid as matrix showed two related ions for the same polypeptide chain, M+2H⁺ = 29338.1 Da and M+H⁺ = 58675 Da.