Cloning, Purification, and Partial Characterization of <i>Bacillus subtilis</i> Urate Oxidase Expressed in <i>Escherichia coli</i>

<table>Thermal stability. Purified enzyme was pre-incubated at <svg height="11.0375" id="M41" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 41.337502 11.0375" width="41.337502" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0375)">
<g transform="translate(72,-63.17)">
<text transform="matrix(1,0,0,-1,-71.95,63.39)">
<tspan style="font-size: 12.50px; " x="0" y="0">−</tspan>
<tspan style="font-size: 12.50px; " x="8.5645552" y="0">2</tspan>
<tspan style="font-size: 12.50px; " x="14.816055" y="0">0</tspan>
</text>
<text transform="matrix(1,0,0,-1,-50.88,68.56)">
<tspan style="font-size: 8.75px; " x="0" y="0">∘</tspan>
</text>
<text transform="matrix(1,0,0,-1,-47.32,63.39)">
<tspan style="font-size: 12.50px; " x="0" y="0">C</tspan>
</text>
</g>
</g>
</svg>, <svg height="11.0375" id="M42" style="vertical-align:-0.17554pt" version="1.1" viewbox="0 0 22.8125 11.0375" width="22.8125" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0375)">
<g transform="translate(72,-63.17)">
<text transform="matrix(1,0,0,-1,-71.95,63.39)">
<tspan style="font-size: 12.50px; " x="0" y="0">4</tspan>
</text>
<text transform="matrix(1,0,0,-1,-65.7,68.56)">
<tspan style="font-size: 8.75px; " x="0" y="0">∘</tspan>
</text>
<text transform="matrix(1,0,0,-1,-62.14,63.39)">
<tspan style="font-size: 12.50px; " x="0" y="0">C</tspan>
</text>
</g>
</g>
</svg> and <svg height="11.0375" id="M43" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 30.625 11.0375" width="30.625" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0375)">
<g transform="translate(72,-63.17)">
<text transform="matrix(1,0,0,-1,-71.95,63.39)">
<tspan style="font-size: 12.50px; " x="0" y="0">3</tspan>
<tspan style="font-size: 12.50px; " x="6.2515001" y="0">7</tspan>
</text>
<text transform="matrix(1,0,0,-1,-59.45,68.56)">
<tspan style="font-size: 8.75px; " x="0" y="0">∘</tspan>
</text>
<text transform="matrix(1,0,0,-1,-55.89,63.39)">
<tspan style="font-size: 12.50px; " x="0" y="0">C</tspan>
</text>
</g>
</g>
</svg> for the indicated times, and assayed at <svg height="11.0375" id="M44" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 30.625 11.0375" width="30.625" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0375)">
<g transform="translate(72,-63.17)">
<text transform="matrix(1,0,0,-1,-71.95,63.39)">
<tspan style="font-size: 12.50px; " x="0" y="0">3</tspan>
<tspan style="font-size: 12.50px; " x="6.2515001" y="0">7</tspan>
</text>
<text transform="matrix(1,0,0,-1,-59.45,68.56)">
<tspan style="font-size: 8.75px; " x="0" y="0">∘</tspan>
</text>
<text transform="matrix(1,0,0,-1,-55.89,63.39)">
<tspan style="font-size: 12.50px; " x="0" y="0">C</tspan>
</text>
</g>
</g>
</svg>. Residual activity corresponds to the percentage of activity determined for the nonincubated samples<b>.</b></table>

BioMed Research International

fig5

Figure 5

Figure 5: Cloning, Purification, and Partial Characterization of <i>Bacillus subtilis</i> Urate Oxidase Expressed in <i>Escherichia coli</i> 

BioMed Research International

Cloning, Purification, and Partial Characterization of Bacillus subtilis Urate Oxidase Expressed in Escherichia coli

Figure 5