Cloning, Purification, and Partial Characterization of Bacillus subtilis Urate Oxidase Expressed in Escherichia coli

Table 1

Summary of the purification steps.

Step	Total activity	Total protein	Specific activity	Purification	Yield
	(IU)	(mg)	(IU/mg)	(fold)	(%)
Crude Extract	54.0	3.0	18.0	1	100
Ni-NTA	31.2	0.8	39.0	2.1	56