(a) B6 alloAg-pulsed, CD80 lenti- and CD86 lenti-transduced DCs were inferior stimulators of naïve syngeneic C3H T cells, compared to alloAg-pulsed NC lenti-transduced DCs or no treated DCs. * compared to NC lenti/No treated. (b) DCs loaded with debris B6 spleen cells were injected into syngeneic C3H mice (/mouse). Seven days later, recipients were sacrificed and splenic T cells challenged ex vivo with -irradiated C3H DCs pulsed with Ag (BALB/c, C3H, or B6 splenocyte lysates). Data are expressed as cpm 1SD from triplicate cultures and are representative of four separate experiments.
