The Role of Genetic Variation Near Interferon-Kappa in Systemic Lupus Erythematosus

Figure 2

Stimulation of WISH reporter cells with IFN-𝛼, IFN-𝜅, and SLE sera with and without anti-IFN-𝜅 antibodies. WISH cells were assayed for type I IFN-induced gene expression as described in the methods, using the stimuli indicated on the graph. Anti-IFN-𝜅 antibody was used at a concentration of 10 𝜇g/mL, and anti-IFN-𝜅 treated sera were preincubated with the antibody for 30 minutes before the sera was applied to the WISH cells. 𝑌-axis shows the IFN-induced gene expression score calculated as described in the Methods.