Oral Vaccination with the Porcine Rotavirus VP4 Outer Capsid Protein Expressed by Lactococcus lactis Induces Specific Antibody Production

Figure 2

SDS-PAGE and western blot analysis. Total cell lysates were analyzed by SDS-PAGE and western blot. Coomassie blue gel staining shows the expression of a 27 KD protein in pNZ8112-VP4/NZ9000 induced by nisin (lane 2, as arrow indicates the VP4 protein), and no corresponding protein in non-nisin induced pNZ8112-VP4/NZ9000 (lane 3) and nisin-induced pNZ8112/NZ9000 (lane 4) can be seen. Similar results were observed by western blot. Nisin-induced pNZ8112-VP4/NZ9000 (lane 5), non-nisin-induced pNZ8112-VP4/NZ9000 (lane 6), and nisin-induced pNZ8112/NZ9000 (lane 7), lane 1, Protein Molecular Marker.