Oral Vaccination with the Porcine Rotavirus VP4 Outer Capsid Protein Expressed by Lactococcus lactis Induces Specific Antibody Production
Figure 3
Cell surface expression of VP4. Nisin-induced pNZ8112-VP4/NZ9000 showed specific green fluorescence when stained either with FITC-conjugated goat anti-mouse IgG containing 1% Evans blue dye (a) or FITC-conjugated goat anti-mouse IgG without Evans blue dye (c). No green fluorescence was observed, but red pellets can be seen in induced pNZ8112-VP4/NZ9000 (d) stained with 1% Evans blue and in pNZ8112-/NZ9000 stained with FITC-conjugated goat anti-mouse IgG containing 1% Evans (b).
