Oral Vaccination with the Porcine Rotavirus VP4 Outer Capsid Protein Expressed by Lactococcus lactis Induces Specific Antibody Production
Figure 5
Anti-VP4-specific IgA responses. Specific anti-VP4 IgA was measured in fecal (a), vaginal washes (b), or lacrimal fluids (c). Mice were orally immunized with 109 CFU of pNZ8112-VP4/NZ9000 and pNZ8112-VP4/NZ9000, respectively. The immune protocol was administered on three consecutive days: immunization at days 0, 1, and 2; a booster at days 14, 15, and 16; and a second booster at days 28, 29, and 30 (the arrows on the -axis indicate the times of vaccination and boosters). Samples from fecal extracts, vaginal washes, and lacrimal fluids were analyzed for the presence of specific Anti-VP4 IgA by ELISA. The OD value of each replicate well is the one of the mixture of two samples; the bars represent IgA mean OD values in four wells representative of eight samples, which were detected at different time points for each group.
