Oral Vaccination with the Porcine Rotavirus VP4 Outer Capsid Protein Expressed by Lactococcus lactis Induces Specific Antibody Production

Table 1

Spleen cell proliferation to VP4 in immunized mic𝑒^𝑎.

Antigen dose (𝜇g/mL)	pNZ8112^b	pNZ8112-VP4^c
0.5	0.036^d ± 0.002	0.087^d ± 0.003
5.0	0.058^d ± 0.001	0.971^d ± 0.003
Positive control (5 𝜇g/mL Con A)	0.871 ± 0.003	0.822 ± 0.002
Control group (RPMI 1640)	0.031 ± 0.002	0.026 ± 0.002

^a𝑛=10.
^bSpleen cell proliferation from mice immunized with L. lactis harboring the pNZ8112 plasmid.
^cSpleen cell proliferation from mice immunized with L. lactis harboring the pNZ8112-VP4 plasmid.
^d𝑃<.05 versus control (𝑡-test).