Acanthopanax koreanum Fruit Waste Inhibits Lipopolysaccharide-Induced Production of Nitric Oxide and Prostaglandin E₂ in RAW 264.7 Macrophages

Figure 6

Effects of CH₂Cl₂ fraction from A. koreanum on the degradation of IκB-α in LPS-stimulated RAW 264.7 cells. RAW 264.7 cells (1.0 × 10⁶ cells/mL) were stimulated with LPS (1 𝜇g/mL) in the presence of A. koreanum (12.5, 25, 50, 100 𝜇g/mL) for 15 minutes. As a control, we also applied PDTC (25 𝜇M), a specific NF-𝜅B inhibitor. Whole cell lysates (25 𝜇g) were prepared and the protein was subjected to 12% SDS-PAGE. Expression of I𝜅B-𝛼 and 𝛽-actin were determined by western blotting. 𝛽-Actin served as a loading control.