A Novel Lectin with Antiproliferative and HIV-1 Reverse Transcriptase Inhibitory Activities from Dried Fruiting Bodies of the Monkey Head Mushroom Hericium erinaceum
Figure 1
Elution profiles of HEA. (a) Anion exchange chromatography of protein derived from the (NH4)2SO4 precipitate of crude extract of H. erinaceum fruiting bodies on a DEAE-cellulose column (). which was equilibrated and eluted with 10 mM phosphate-buffered (pH 7.0). Arrows indicate elution of the column stepwise with 50 mM, 300 mM and 1000 mM NaCl in the starting buffer. (b) Ion exchange chromatography of D3 on a CM-cellulose column (). Arrows indicate elution of the column stepwise with 50 mM, 150 mM and 1000 mM NaCl in 10 mM NH4OAc buffer (pH 5.1). (c) Ion exchange chromatography of fraction C2 on a Q-Sepharose column (). The slanting line across the chromatogram denotes a linear NaCl concentration (0 to 400 mM) gradient in 10 mM NH4OAc buffer (pH 5.1). (d) Gel filtration of fraction Q3 from Q-Sepharose column on a Superdex G-75 column, which was eluted with 10 mM phosphate buffer (pH 7.5) containing 150 mM NaCl. The flow rate was 0.4 ml/min.
