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Journal of Biomedicine and Biotechnology
Volume 2010, Article ID 734898, 13 pages
Research Article

Identification of Four Entamoeba histolytica Organellar DNA Polymerases of the Family B and Cellular Localization of the Ehodp1 Gene and EhODP1 Protein

1Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México, San Lorenzo 290, Col. Del Valle, CP 03100 México, DF, Mexico
2Departmento de Biología Celular, Cinvestav-IPN, Av. IPN 2508, Col. Zacatenco, CP 07360 México, DF, Mexico
3Departmento de Infectómica y Patogénesis Molecular, Cinvestav-IPN, Av. IPN 2508, Col. Zacatenco, CP 07360 México, DF, Mexico

Received 18 August 2009; Accepted 15 December 2009

Academic Editor: Isaac K. O. Cann

Copyright © 2010 María Esther Herrera-Aguirre et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We report the identification of a family of four active genes (Ehodp1, Ehodp2, Ehodp3, and Ehodp4) encoding putative DNA polymerases in Entamoeba histolytica, the protozoan parasite responsible of human amoebiasis. The four Ehodp genes show similarity to DNA polymerases encoded in fungi and plant mitochondrial plasmids. EhODP polypeptides conserve the exonuclease II and polymerization domains, and they have the I, II, and III conserved boxes that characterize them as DNA polymerases of family B. Furthermore, we found in EhODP polymerases two novel A and B boxes, present also in DNA polymerases encoded in fungi mitochondrial plasmids. By in situ PCR, Ehodp1 gene was located in nuclei and in DNA-containing cytoplasmic structures. Additionally, using polyclonal antibodies against a recombinant rEhODP1-168 polypeptide, and confocal microscopy, EhODP1 was located in cytoplasmic DNA-containing structures.