Research Article

Identification of Four Entamoeba histolytica Organellar DNA Polymerases of the Family B and Cellular Localization of the Ehodp1 Gene and EhODP1 Protein

Figure 1

Alignment of the E. histolytica putative organellar DNA polymerases. The five conserved boxes I, II, III, A and B are framed. Identical amino acids are shown in black. Conserved changes are shown in grey. Numbers indicate the positions of amino acids in each polypeptide. Arrows locate the region used to design specific oligonucleotide pairs to amplify each gene by RT-PCR, and numbers 1, 2, 3, and 4 correspond to Ehodp1, Ehodp2, Ehodp3 and Ehodp4 genes, respectively. Putative DNA_pol_B_2 (PF03175) domain (described for organellar and viral DNA polymerases of family B) is underlined. Exonuclease II domain is indicated with a dotted underlining. Arrowheads indicate the aspartic acid residues that are required for the catalytic activity.
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