Table of Contents Author Guidelines Submit a Manuscript
Journal of Biomedicine and Biotechnology
Volume 2010, Article ID 737289, 8 pages
http://dx.doi.org/10.1155/2010/737289
Research Article

Biomolecular Interaction Study of Cyclolinopeptide A with Human Serum Albumin

1Department of Biochemistry, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E5
2Department of Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, SK, Canada S7N 5A8
3Saskatchewan Structural Sciences Centre, University of Saskatchewan, Saskatoon, SK, Canada S7N 5C9
4Department Plant Sciences, University of Saskatchewan, Saskatoon, SK, Canada S7N 5A8

Received 31 August 2010; Revised 4 December 2010; Accepted 21 December 2010

Academic Editor: Abdel A. Abdel-Rahman

Copyright © 2010 Ben Rempel et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The kinetics, energetics, and structure of Cyclolinopeptide A binding with Human Serum Albumin were investigated with surface plasmon resonance and circular dichroism. The complex is formed through slow recognition kinetics that is temperature sensitive in the range of 20°C–37°C. The overall reaction was observed to be endothermic ( kJ ) and entropy driven ( J ) with overall small changes to the tertiary structure.