Depletion of DNMT3A Suppressed Cell Proliferation and Restored PTEN in Hepatocellular Carcinoma Cell
DNMT3A gene expression is inhibited by siRNA of DNMT3A in SMMC-7721 cells. (a) Schematic representation of DNMT3A mRNA structure and siRNA positions is showed. (b) Western blot analysis efficiency of DNMT3A siRNA, β-actin was used as reference control. Lanes 1–3 indicated the cell line transfected with plasmid pSUPER-EGFP, sMT3A, and pMT3A, respectively. Right: the intensity ratios of both target gene band and control from the same sample were determined and calculated by Gel-Pro Analyzer 3.0. (c) RT-PCR evaluated the affect of DNMT3A-specific siRNA on other DNMTs expression. (d) Real-time quantitative RT-PCR confirmed the efficiency of DNMT3A-specific siRNA and the affect on other DNMTs mRNA expression.
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