Depletion of DNMT3A Suppressed Cell Proliferation and Restored PTEN in Hepatocellular Carcinoma Cell
Depletion of DNMT3A suppressed HCC cell growth and colony formation ability. (a) Expression of DNMT3A was stably suppressed in SMMC-7721. 7721-sMT3A, which is transfected with scramble vector sMT3A, 7721-pMT3A, which is transfected with candidate DNMT3A siRNA constructs. (b) Colony formation of SMMC-7721 cells was inhibited when transfected with DNMT3A siRNA. The scramble vector served as a control. The number of colonies was counted from three independent experiments. The histogram shows the colony formation efficiency, where the numbers represent the average value of three independent experiments.(c) Cell growth curve of SMMC-7721 cells treated with the DNMT3A siRNA. The cells transfected with the scramble vector served as control. The experiments were repeated at least three times. The result represents the average value of triplicate wells +/- the standard deviation.
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