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Journal of Biomedicine and Biotechnology
Volume 2010 (2010), Article ID 737535, 10 pages
Research Article

Depletion of DNMT3A Suppressed Cell Proliferation and Restored PTEN in Hepatocellular Carcinoma Cell

1Key Laboratory of Developmental Genes and Human Diseases, Ministry of Education, Southeast University, Nanjing 210009, China
2Institute of Life Science, Southeast University, Nanjing 210009, China
3Prenatal Diagnosis Center, Nanjing Maternity and Child Health Care Hospital, Nanjing 210009, China

Received 24 November 2009; Revised 25 February 2010; Accepted 26 February 2010

Academic Editor: Mouldy Sioud

Copyright © 2010 Zhujiang Zhao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary materials include two tables, one is about candidate DNMT3A siRNA sequences (19nt) for knocking down DNMT3A in HCC cell lines, the other is about 153 genes up-regulated in DNMT3A knockdown cell.

Supplementary materials also provided the depletion of DNMT3A in QSG-7701 cells to perform the same experiments, including RNA interference, cell proliferation, colony formation and PTEN expression.

  1. Supplementary Figure
  2. Supplementary Table