Figure 4: Size distribution of FSCPs. (a) Fish scale hydrolysates were separated by gel chromatography (superdex peptide 10/300 GL columns); molecular sizes were determined for each fraction; size distributions were plotted accordingly. The size distribution (KDa) is expressed as means ± SEM. (b) Size profiles of FSCPs. Protein standards (Bio-Rad, California, USA) are cytochrome C (Mr = 12500 Da), aprotinin (Mr = 6512 Da), vitamin B12 (Mr = 1355 Da), and cytidine (Mr = 246 Da). The average molecular weights (MWs) were determined to be 4500, 3500, 2000, and 1300 Da for the four indicated groups.