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Journal of Biomedicine and Biotechnology
Volume 2010 (2010), Article ID 781365, 18 pages
http://dx.doi.org/10.1155/2010/781365
Research Article

Transcriptome Analysis of the Phytobacterium Xylella fastidiosa Growing under Xylem-Based Chemical Conditions

1Núcleo Integrado de Biotecnologia, Universidade de Mogi das Cruzes, Avenida Dr. Cândido Xavier de Almeida Souza 200, Mogi das Cruzes 08780-911 SP, Brazil
2Centro Interdisciplinar de Investigação Bioquímica, Universidade de Mogi das Cruzes, Avenida Dr. Cândido Xavier de Almeida Souza 200, Mogi das Cruzes 08780-911 SP, Brazil

Received 25 November 2009; Revised 15 March 2010; Accepted 12 April 2010

Academic Editor: Marco Bazzicalupo

Copyright © 2010 Maristela Boaceff Ciraulo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Xylella fastidiosa is a xylem-limited bacterium responsible for important plant diseases, like citrus-variegated chlorosis (CVC) and grapevine Pierce's disease (PD). Interestingly, in vitro growth of X. fastidiosa in chemically defined media that resemble xylem fluid has been achieved, allowing studies of metabolic processes used by xylem-dwelling bacteria to thrive in such nutrient-poor conditions. Thus, we performed microarray hybridizations to compare transcriptomes of X. fastidiosa cells grown in 3G10-R, a medium that resembles grape sap, and in Periwinkle Wilt (PW), the complex medium traditionally used to cultivate X. fastidiosa. We identified 299 transcripts modulated in response to growth in these media. Some 3G10R-overexpressed genes have been shown to be upregulated in cells directly isolated from infected plants and may be involved in plant colonization, virulence and environmental competition. In contrast, cells cultivated in PW show a metabolic switch associated with increased aerobic respiration and enhanced bacterial growth rates.