Transcriptome Analysis of the Phytobacterium Xylella fastidiosa Growing under Xylem-Based Chemical Conditions
Figure 2
Evaluation of transcriptional modulation of selected genes by Real-Time qPCR. In order to confirm the reliability of the microarray experiments, 16 genes have been randomly selected and their transcription modulation was verified by Real-Time qPCR. The same RNA samples used in the microarray hybridizations were converted to cDNA and the relative expression ratios (RQ) of these genes have been measured with the aid of specific Taq-Man probes. ORF Xf1311, which encodes a rod-shaped determining protein (MreD), has been used as an endogenous control for experimental normalization, since the microarray hybridization experiments showed that this ORF is constitutively expressed in both PW and 3G10R. Variations in transcriptional modulation were calculated having the expression levels in PW as a reference and are represented by the log2 ratio of the relative quantifications (RQ). Experiments were performed in triplicate and graphic shows the average values and their respective standard deviations.
