Research Article

Transcriptome Analysis of the Phytobacterium Xylella fastidiosa Growing under Xylem-Based Chemical Conditions

Figure 3

Evaluation of respiratory rates in Xylella fastidiosa cells growing in PW and 3G10R. X. fastidiosa cells were grown into middle exponential phase in PW and subsequently transferred (in a 1 : 20 proportion) into fresh PW and 3G10R cultures. Bacterial growth in both cultures was monitored through O D 6 0 0 measurements and aliquots were taken from each culture to evaluate O 2 consumption with the aid of an oxygraph in intact cells. Respiratory rate for each culture was calculated as the ratio between O 2 consumption and the respective O D 6 0 0 value obtained at each time point. Measurements were taken until day 7 (in PW) and day 13 (in 3G10R). Experiments were performed in triplicate and graphic shows the average values and their respective standard deviations.
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