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Journal of Biomedicine and Biotechnology
Volume 2010, Article ID 790247, 9 pages
http://dx.doi.org/10.1155/2010/790247
Research Article

A Novel Negative Fe-Deficiency-Responsive Element and a TGGCA-Type-Like FeRE Control the Expression of FTR1 in Chlamydomonas reinhardtii

1Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou 571101, China
2Department of Biochemistry, Hainan Medical College, Haikou 571101, China
3Umeå Plant Science Center, Umeå University, 901 87 Umeå, Sweden

Received 13 October 2009; Accepted 30 December 2009

Academic Editor: Gopi K. Podila

Copyright © 2010 Xiaowen Fei et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We have reported three Fe-deficiency-responsive elements (FEREs), FOX1, ATX1, and FEA1, all of which are positive regulatory elements in response to iron deficiency in Chlamydomonas reinhardtii. Here we describe FTR1, another iron regulated gene and mutational analysis of its promoter. Our results reveal that the FeREs of FTR1 distinguish itself from other iron response elements by containing both negative and positive regulatory regions. In FTR1, the 291/ 236 region from the transcriptional start site is necessary and sufficient for Fe-deficiency-inducible expression. This region contains two positive FeREs with a TGGCA-like core sequence: the FtrFeRE1 (ATGCAGGCT) at 287/ 279 and the FtrFeRE2 (AAGCGATTGCCAGAGCGC) at 253/ 236. Furthermore, we identified a novel FERE, FtrFeRE3 (AGTAACTGTTAAGCC) localized at 319/ 292, which negatively influences the expression of FTR1.