A Novel Negative Fe-Deficiency-Responsive Element and a TGGCA-Type-Like FeRE Control the Expression of FTR1 in Chlamydomonas reinhardtii

Table 1

The comparison of Ars mRNA levels and Ars activities of selective transformants.

Constructs	Ars mRNA Abundances			Ars Activities (nmol p-nitrophenol × min−1 × 10−6 cells)
	−Fe (0𝜇M)	+Fe (18 μM)	−Fe/+Fe	−Fe (0𝜇M)	+Fe (18𝜇M)	−Fe/+Fe
Ftr1179	115	22	5	2.97	0.58	5
Ftr291	372	36	10	6.86	0.76	9
Ftr253	2	2	1	0.31	0.32	1
319–194	1	1	1	0.29	0.26	1
291–194	133	26	5	3.49	0.55	6
FtrM291F	186	29	6	5.41	0.81	7
FtrM287F	3	2	2	0.33	0.29	1

Ars mRNA levels were assessed by real-time PCR in −Fe (0𝜇M) and +Fe (18 μM) conditions. Data were calculated by the 2−ΔΔCTmethod [29]. The Ars activities of the selected transformants were detected as described by Davies et al. [42].