A Novel Negative Fe-Deficiency-Responsive Element and a TGGCA-Type-Like FeRE Control the Expression of FTR1 in Chlamydomonas reinhardtii

Table 2

Primers used in amplification of the fragments for making the deletion constructs.

Constructs	5Primers	3Primers	The position
Ftr1179	1179F TCGGTACCTTACTGGCTTACTGGCA	58R GAAGTCGAC CGTCCTGATGAGAAAGG	−1179/+58
Ftr917	917F TAAGGTACCGAACCTAACAAATGGCA	58R GAAGTCGAC CGTCCTGATGAGAAAGG	−917/+58
Ftr718	718F TAAGGTACCCCAAGATCACCCGTCTG	58R GAAGTCGAC CGTCCTGATGAGAAAGG	−718/+58
Ftr511	511F CTTGGTACCTTCCAGCCCGACTGAAT	58R GAAGTCGAC CGTCCTGATGAGAAAGG	−511/+58
Ftr355	355F ATTGGTACCCGACACACCTTGCTCCA	58R GAAGTCGAC CGTCCTGATGAGAAAGG	−355/+58
Ftr291	291F GACGGTACCTCACATGCAGGCTCCTCCT	58R GAAGTCGAC CGTCCTGATGAGAAAGG	−291/+58
Ftr253	253F TAAGGTACCAAGCGATTGCCAGAGCG	58R GAAGTCGAC CGTCCTGATGAGAAAGG	−253/+58
Ftr161	161F TAAGGTACCATGCCACCGATTACGAG	58R GAAGTCGAC CGTCCTGATGAGAAAGG	−161/+58
Ftr59	59F TAAGGTACCGGAGCGACGGTAAACGG	58R GAAGTCGAC CGTCCTGATGAGAAAGG	−59/+58
Ftr24	24F ATCGGTACCATATAGCACTCTAAC	58R GAAGTCGAC CGTCCTGATGAGAAAGG	−24/+58
355−162	355F GACGGTACCCGACACACCTTGCTCCACCG	162R GACGGTACCGCATGCGTTCTCTTATTG	−355/−162
355−194	355F GACGGTACCCGACACACCTTGCTCCACCG	194R GACGGTACCGACATTATCAGCTAGCG	−355/−194
334−194	334F GACGGTACCGAGCGAGGCTACTGGTC	194R GACGGTACCGACATTATCAGCTAGCG	−334/−194
291−162	291F GACGGTACCTCACATGCAGGCTCCTCCT	162R GACGGTACCGCATGCGTTCTCTTATTG	−291/−162
291−194	291F GACGGTACCTCACATGCAGGCTCCTCCT	194R GACGGTACCGACATTATCAGCTAGCG	−291/−194
291−215	291F GACGGTACCTCACATGCAGGCTCCTCCT	215R GACGGTACCCAGTGCATG GCGACAGCC	−291/−215
291−236	291F GACGGTACCTCACATGCAGGCTCCTCCT	236R GACGGTACCGCGCTCTG GCAATCGCTTC	−291/−236
291−254	291F GACGGTACCTCACATGCAGGCTCCTCCT	254R GACGGTACCCGCGCGTGCCCGGTCGG	−291/−254
FtrD5(overlap	A,1179F AAGGTACCGAACCTAACAAATGGCA	B,291R GCAATCGCTTGGCTTAACAGTTACTG	−1179/+58 with
extension PCR)	C,253F CTGTTAAGCCAAGCGATTGCCAGAG	D,58R GAAGTCGACCGTCCTGATGAGAAAGG	−291/−254 removal