Multiple-Locus Variable-Number Tandem Repeat Analysis of Vibrio cholerae in Comparison with Pulsed Field Gel Electrophoresis and Virulotyping

Table 1

Characteristics of V. cholerae VNTR loci.

Character	Primers sequence	Size range of PCR products (bp)	VNTR position (kb)	Alleles	D index
VCTR 1	F: 5^-GAGAAAAGCCAAACCACTGC-3^	212–230	137	9	0.82
	R: 5^-TTTAAGCGCGCAAAGAAACT-3^
VCTR 2	F: 5^-CGTTAGCATCGAAACTGCTG-3^	216–252	467	5	0.75
	R: 5^-CCACTCAATCTCGTGGGAAA-3^
VCTR 3	F: 5^-AGTGGGCACAGAGTGTCAAA-3^	218–276	1778	7	0.84
	R: 5^-GCTGTACTCTGGCACATCCA-3^
VCTR 4	F: 5^-TCGAATGTATGGGGAACATTT-3^	278–300	303∗	17	0.92
	R: 5^-TTAATACCCCTTTCTTCCGATG-3^
VCTR 5	F: 5^-GAAGAGACGGACCTTGATCG-3^	228–251	1685	6	0.65
	R: 5^-TCGCAGCAAGTTTGCTTAAC-3^
VCTR 6	F: 5^-GTTTTATCGCTGATGCGTGA-3^	211–269	540∗	4	0.59
	R: 5^-GCAAGCAAGTGCCGAATTAT-3^
VCTR 7	F: 5^-GTTTGAGAGCTCGCCTCTTG-3^	328–369	187∗	11	0.89
	R: 5^-CAATCTCGCCAATGCTTATG-3^

∗position in chromosome II of 𝑉.𝑐ℎ𝑜𝑙𝑒𝑟𝑎𝑒 genom.