Multiple-Locus Variable-Number Tandem Repeat Analysis of Vibrio cholerae in Comparison with Pulsed Field Gel Electrophoresis and Virulotyping
Table 1
Characteristics of V. cholerae VNTR loci.
Character
Primers sequence
Size range of PCR products (bp)
VNTR position (kb)
Alleles
D index
VCTR 1
F: -GAGAAAAGCCAAACCACTGC-
212–230
137
9
0.82
R: -TTTAAGCGCGCAAAGAAACT-
VCTR 2
F: -CGTTAGCATCGAAACTGCTG-
216–252
467
5
0.75
R: -CCACTCAATCTCGTGGGAAA-
VCTR 3
F: -AGTGGGCACAGAGTGTCAAA-
218–276
1778
7
0.84
R: -GCTGTACTCTGGCACATCCA-
VCTR 4
F: -TCGAATGTATGGGGAACATTT-
278–300
303
17
0.92
R: -TTAATACCCCTTTCTTCCGATG-
VCTR 5
F: -GAAGAGACGGACCTTGATCG-
228–251
1685
6
0.65
R: -TCGCAGCAAGTTTGCTTAAC-
VCTR 6
F: -GTTTTATCGCTGATGCGTGA-
211–269
540
4
0.59
R: -GCAAGCAAGTGCCGAATTAT-
VCTR 7
F: -GTTTGAGAGCTCGCCTCTTG-
328–369
187
11
0.89
R: -CAATCTCGCCAATGCTTATG-
position in chromosome II of genom.
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