RNAi-Based Strategies for Cyclooxygenase-2 Inhibition in Cancer
Figure 1
COX-2 silencing mediated by transkingdom RNAi. The cotransformation of E. coli with both plasmids pGB2inv-hly and pSUPER. enables bacteria to induce the transkingdom RNAi phenomenon in colon cancer cells. Following the expression of the bacterial proteins invasin and listeriolysin-O, engineered E. coli strains are able to permeate human cells and release the plasmid DNA content. Anti-COX-2 shRNAs are then transcribed by the cellular machinery, resulting in an efficient COX-2 silencing.
