Proposing Low-Similarity Peptide Vaccines against Mycobacterium tuberculosis

Table 1

Similarity analysis of M. tuberculosis B-cell epitopes to the host proteome.

Epitope Source Name	IEDB ID^1	Epitope Sequence^2	Number of Matches^3	Host Proteome^4	Refs.
10 kDa chaperonin	3293	wdedGEKRIpldvae	4	H	[12, 36]
10 kDa chaperonin	6508	PDTAKekpqegtvva^5	3	H	[12, 36]
10 kDa chaperonin	3294	ldvaegdtvIYSKY^5	2	H	[12, 36]
10 kDa chaperonin	1011756	gtvvavgpGRWDEdge^5	3	H	[37–39]
10 kDa chaperonin	1011758	GRWDEdgekripldva	3	H	[37–39]
10 kDa chaperonin	3290	etttasglviPDTAK^5	3	H	[12, 36, 38]
10 kDa chaperonin	3289	dkilvQANEAettta	1	H	[12, 36]
6 kDa ESAT^5	8105	yqgvQQKWD^5	1	M	[40]
6 kDa ESAT	8086	eQQWNFagieaaa^5	0	M	[40]
6 kDa ESAT	8090	DEGKQsltk^5	2	M	[40]
6 kDa ESAT	8106	aaAWGGSgsEAYQGvQQKWData^5	3, 1, 1	M	[40]
DNAK	1052559	lvyQTEKF	4	H	[41]
DNAK	1052560	vyQTEKFv	4	H	[41]
DNAK	1052558	yQTEKFvk	4	H	[41]
DNAK	1052561	qTEKFVke	3	H	[41]
DNAK	1052562	TEKFVkeq	3	H	[41]
DNAK	1052583	lNKVDAav	4	H	[41]
DNAK	1052584	NKVDAava	4	H	[41]
DNAK	1052589	aeaEGGTW	3	H	[41]
DNAK	1052591	aegGTWRI	2	H	[41]
DNAK	1052593	ggTWRIGY	0	H	[41]
DNAK	1052594	WRIGYfgh	0	H	[41]
DNAK	1052595	riGYFGHq	2	H	[41]
DNAK	1052596	igYFGHQv	1	H	[41]
DNAK	1052597	GYFGHqvg	2	H	[41]
DNAK	1052598	YFGHQvgd	1	H	[41]
DNAK	24658	HQVGDgea	1	H	[41]
DNAK	1052627	lrsSSGCV	5	H	[41]
DNAK	1052628	rssSGCVT	4	H	[41]
DNAK	1052629	sssGCVTG	4	H	[41]
DNAK	1052630	ssgCVTGH	1	H	[41]
DNAK	1052631	cvTGHWRc	0	H	[41]
DNAK	1052632	VTGHWrcp	0	H	[41]
DNAK	1052633	TGHWRCpp	0	H	[41]
DNAK	1052549	HWRCPprr	1	H	[41]
DNAK	1052550	WRCPPrrr	1	H	[41]
ESAT-6-like protein ESXB	6090	laqeagNFERIsgdl^5	2	H	[42]
ESAT-6-like protein ESXB	6090	laqeagNFERIsgdl	1	M	[42]
Immunogenic protein MPT64	1043665	nitsATYQSaipprgtqavv	1, 0	H	[43]
Immunogenic protein MPT64	1043668	hptttyKAFDWdqayrkpit	0	H	[43]
Lipoprotein LPQH	1034449	vtgsvvCTTAAgnvniaigg^5	2	M	[44]
Lipoprotein LPQH	1107625	vtGSVVC^5	5	M	[44]
Lipoprotein LPQH	1107627	VNKSFei	1	M	[44]
Lipoprotein LPQH	1052656	VKRGLtvavagaailvagls	2	M	[45]
Lipoprotein LPQH	1034455	taspgaasgpkvvidGKDQN	4	M	[44]
Lipoprotein LPQH	1018364	dMANPMspvnksfeievtcs	0	M	[44]
MTP40 protein	1013654	gprlyGEMTMqgtrkprpsgp	2	H	[46]
MTP40 protein	1011740	ttlGMHCGsfgsapsng	0	H	[46]
MTP40 protein	1011751	mlgtGTPNRarINFNC	3, 3	H	[46]
MTP40 protein	1011736	MLGNApsvvpnTTLGM	4, 3	H	[46]
MTP40 protein	1011754	INFNCevWSNVSetisgprly	3, 2	H	[46]
Phosphate-binding protein 1	1127065	lfnlWGPAFherypnvtita	1	M	[44]
Phosphate-binding protein 1	1019382	kqdpegWGKSPgfgttvdfp	1	M	[47]
Phosphate-binding protein 1	1107628	GPAFHer	1	M	[44]
Phosphate-binding protein 1	1123943	gnggMVTGCaetpgcvayig	1	M	[48]
Phosphate-binding protein 1	1107630	gfasKTPANqais	1	M	[44]

¹IEDB ID Reference Dataset of Mycobacterial Immune Epitopes: http://iedb.zendesk.com/entries/18171-reference-datasets-of-mycobacterial-immune-epitopes.
²Epitope sequence with amino acid sequence in one-letter code. Low-similarity pentapeptides in capital letters.
³Number of times the low-similarity pentapeptide fragment(s) occur(s) in the set of proteins that comprehensively form the host proteome (see under Methods and [17–27]).
⁴Host proteome: H, human; M, murine.
⁵Sequences entirely or partially contained in M. tuberculosis T-cell epitopes [36, 49–52].
⁶ESAT, early secretory antigenic target.