Preparation and Characterization of a Novel Skin Substitute
Figure 2
The dermis basement membrane obtained by the three different techniques stained with Collagen IV MoAb. (a) Dispase II, (b) Trypsin, (c) the manual method. Only the manual method left an integral structure. Arrows indicate the absence of basement membrane. Original magnification: 10x. AAGD: alloplastic acellular glycerolized dermis; BM: basement membrane.
