Figure 4: Histomorphological analysis performed on the de-epithelialized dermis to verify the integrity of the tissue architecture and determine the cell presence over time. Sections were stained with Mayer’s hematoxylin solution. (a) at day 0, after the glycerol removal and before the de-epithelialization: arrows indicate the presence of infiltrating cells; (b) at 2 days, (c) at 7 days, (d) at 14 days. The number of ghost cells decrease constantly over time and are no longer present as from 7 days. Original magnification: 10x. E: epidermis, De: dermis, AAGD: alloplastic acellular glycerolized dermis.