Size of Insertions in Promoter Region Modulates Gmhsp17.6-L mRNA Transcript Levels
Figure 6
Ribonuclease protection assay results showing the expression of Gmhsp176-L mRNA transcripts. Figure shows susceptible parental BRS133 in inoculated and non-inoculated treatments, respectively and resistant parental PI595099, in inoculated and non-inoculated treatments, respectively. Also, a positive control provided with the RPA kit, a 250 pb Kpn l-Xba l fragment of the mouse -actin gene subcloned from pAL41 is showed and probes control, a positive digestion control and a probe-negative digestion control. Black box indicates the bands and white arrow the positive control band.
