Research Article

Size of Insertions in Promoter Region Modulates Gmhsp17.6-L mRNA Transcript Levels

Figure 8

Relative quantification of Gmhsp176-L mRNA expression levels ( 𝑥 ), in resistant (JF7002, JF7027 and JF7056) and susceptible (256-S, 259-S and 266-S) offsprings, in inoculated and non-inoculated treatments. Data were obtained by real-time PCR with primers SoyHsp PSC-F and SoyHsp PSC-R, designed using Primer Express Software v20 package and the entire Gmhsp176-L gene sequence available in Genbank (Accession no. M11317). All analyses were carried out using SDS software package. As a calibrator, value 1X, the susceptible individual 256-S was chosen, which presented in the promoter alignment smallest AT(n) repetition.