The Investigation of Effects of Quercetin and Its Combination with Cisplatin on Malignant Mesothelioma Cells In Vitro
Figure 4
SPC212 ((a) and (b)) and SPC111 cells ((c) and (d)) were treated with 5 g/mL CIS, 10 g/mL CIS, 50 M QU, 5 g/mL CIS 50 M QU, and 10 g/mL CIS 50 M QU for 48 hours. C9 ((a) and (c)) and C3 ((b) and (d)) activities were measured as indicated in Section 2.5 compared to control and single agent treatments. 10 g/mL CIS 50 M QU combinations activated C9 in both cell lines for 48 hours ((a) and (c)). C9 and C3 activities were increased in SPC212 cells following 10 g/mL CIS 50 M QU at 24 hours when compared to the control cells and the cells treated with individual doses of QU and CIS ((a) and (b)). Additionally, 5 g/mL CIS 50 M QU combination treatments of SPC212 cells were found effective to activate C9 and C3 molecules at 48 hours ((a) and (b)). In addition C9 was activated with 10 g/mL CIS 50 M QU applications on SPC111 cells. However, there was no C3 activity detected in SPC111 cells for 48 hours (d). Data was statistically evaluated between control group, single dose groups and combination dose groups, with the level of significance chosen as , , between QU CIS treatment and CIS alone, and between QU CIS treatment and QU alone.
