BacMam Virus Transduced Cardiomyoblasts Can Be Used for Myocardial Transplantation Using AP-PEG-A Microcapsules: Molecular Cloning, Preparation, and In Vitro Analysis
Figure 5
Cell survival and growth kinetics of the Bac-null transduced cells encapsulated in AP-PEG-A microcapsules. For the encapsulation cells/ml of alginate were used and maintained in DMEM + 10% FBS at 37°C and were incubated for 30 days. Medium was replaced at alternate days. Experimental samples were treated with calcein AM for 1 h, and they were measured using fluorescence plate reading at excitation 485 nm, emission 535 nm.